Bruker TruLive3D Imager Light-Sheet Microscope

(I) Description

The Bruker Luxendo TruLive3D Imager is an inverted light-sheet fluorescence microscope optimized for fast, long-term 3D imaging of delicate live specimens in their native biological environment. Its dual-sided illumination and single-lens detection from below enable rapid acquisition, deep imaging, and uniform illumination with minimal shadowing, while advanced environmental control helps preserve sample viability during experiments lasting from hours to days. A large multiplex sample holder supports hundreds of organoids, spheroids, 3D cell cultures, or small embryos and enables comparison of up to six experimental conditions within a single experiment.

(II) Features & Benefits

Fast, low-phototoxicity 3D imaging of delicate live specimens
Dual-sided illumination for deep, uniform imaging with reduced shadowing
High-NA detection for excellent sensitivity and spatial resolution
Long-term imaging under controlled physiological conditions
Multiplex acquisition of hundreds of samples and up to six experimental conditions
Dual-destriping and TAG-lens (tunable acoustic gradient lens) technologies for improved light-sheet uniformity
Easy open-top sample access and flexible TruLive3D sample holders
Optional photomanipulation for advanced functional imaging experiments
Integrated LuxBundle software for acquisition, processing, and 3D visualization

These capabilities are designed particularly for multi-sample and multi-condition live-imaging workflows involving organoids, spheroids, cell cultures, and small embryos.

(III) Technical Specifications

Imaging modality: Inverted Light-Sheet Fluorescence Microscopy with dual-sided illumination and single-lens detection
Light-sheet generation: Beam-scanned Gaussian light sheets
Light-sheet thickness: Adjustable from 2 to 6 µm
Illumination optics: Chromatically corrected from 440 to 660 nm
Illumination objectives: 2 × Nikon CFI Plan Fluor 10× W, 0.3 NA water-immersion objectives
Detection objective: Nikon CFI Apo 25× W, 1.1 NA water-immersion objective
Alternative objective configuration: Olympus XLUMPLFLN 16×, 0.8 NA
Effective magnification: Multiple configuration-dependent options from approximately 8× to 62.5×
Detection: 2 × Hamamatsu ORCA-Flash4.0 V3 high-speed sCMOS cameras
Camera resolution: 2048 × 2048 pixels with 6.5 × 6.5 µm pixel size
Acquisition speed: More than 80 frames per second at full frame and up to 500 frames per second with subframe cropping
Peak quantum efficiency: Up to 82% at 560 nm
Spectral detection: Two detection channels with 10-position high-speed emission filter wheels
Simultaneous imaging: Three motorized dichroic positions for simultaneous dual-channel acquisition
Illumination control: Motorized neutral-density filters with 90%, 10%, and 1% transmission
Environmental control: Temperature from 20 to 37 °C, CO₂, O₂/N₂, and humidity control
Sample capacity: 75 mm sample holder accommodating up to hundreds of specimens
Experimental conditions: Up to six separate conditions using three dual-compartment TruLive3D dishes
Sample mounting: FEP-foil-based sample holders for cells, organoids, spheroids, and embryos
Stage travel: 66 × 3 × 3 mm
Artifact reduction: Dual-destriping through pivot scanning
Light-sheet optimization: TAG-lens technology for rapid axial scanning and illumination uniformity
Photomanipulation: Optional photomanipulation module
Software: Bruker LuxBundle for acquisition, multidimensional experiment control, post-processing, and 3D visualization
System design: Compact, vibration-free benchtop configuration without the need for an air table

Bruker’s published objective, magnification, and field-of-view values depend on the selected optical configuration; the final configuration should therefore be confirmed when preparing a quotation or formal datasheet.

(IV) Manufacturer: Bruker

(V) Applications

Live-Cell Imaging, Long-Term Time-Lapse Imaging, 3D Cell Culture Imaging, Organoid Imaging, Spheroid Imaging, High-Throughput Multi-Sample Imaging, Multi-Condition Drug Studies, Developmental Biology, Embryo Development, In Vivo Imaging of Small Model Organisms, Zebrafish Research, Drosophila Research, Mouse Embryo Imaging, Cancer Biology, Tumorigenesis, Drug-Response Studies, Toxicology, Stem Cell Research, Primary Cell Imaging, Cell–Cell Interaction Studies, Immunology, Wound-Healing Studies, Photomanipulation.