Bruker InViSPIM Lattice Pro Light-Sheet Microscope

(I) Description:

The Bruker InVi SPIM Lattice Pro is an advanced inverted light-sheet fluorescence microscope engineered for high-resolution imaging of live and fixed biological specimens. Utilizing a spatial light modulator (SLM), the system generates a wide range of illumination beam patterns—including lattice light sheets—allowing the illumination to be optimized for different sample types and experimental applications. Its inverted optical architecture, featuring high-NA illumination and detection objectives together with a fully environmentally controlled imaging chamber, enables long-term imaging under physiological conditions while minimizing photobleaching and phototoxicity. The InVi SPIM Lattice Pro also supports advanced imaging modalities, including structured illumination microscopy (SIM), calcium imaging, fluorescence lifetime imaging microscopy (FLIM), fluorescence correlation spectroscopy (FCS), and photomanipulation, making it a versatile platform for cutting-edge life science research.

(II) Features and benefits:

· High-resolution imaging of live and fixed biological specimens
· Programmable illumination using Spatial Light Modulator (SLM) technology
· Multiple illumination beam patterns, including lattice light sheets, optimized for different sample types
· High-NA illumination and detection optics for superior image quality and sensitivity
· Gentle light-sheet illumination minimizes photobleaching and phototoxicity during long-term imaging
· Fully environmentally controlled imaging chamber for stable physiological conditions
· Supports advanced imaging modalities including SIM, FLIM, FCS, calcium imaging, and photomanipulation
· Flexible platform for demanding live-cell imaging and advanced fluorescence microscopy applications

(III) Technical Specifications:

· Imaging Modality: Inverted Light-Sheet Fluorescence Microscopy (LSFM)
· Illumination technology: Advanced Illumination Module (AIM) with integrated Spatial Light Modulator (SLM)
· Beam patterns: Static Gaussian, Scanned Gaussian, Scanned Bessel, Optical Lattice, and user-defined beam profiles
· Advanced imaging modes: Structured Illumination Microscopy (SIM), FLIM, FCS, calcium imaging, and optional photomanipulation
· Illumination objective: Special Optics 28.6× @ 0.7 NA water immersion objective
· Detection objective: Nikon CFI Apo 25× @ 1.1 NA water immersion objective with correction collar
· Effective magnification: 31,3× și 62,5×
· Detection: Two sCMOS cameras each equipped with up to 10 filters split by two selectable dichroic mirrors.
· Excitation lasers: Up to 6 laser lines with chromatic correction
· Emission filters: Up to 20 selectable emission filters
· Environmental control: Temperature (20–37 °C), CO₂, O₂/N₂, and humidity control for long-term live-cell imaging
· Software: Bruker LuxBundle for acquisition, image reconstruction, visualization, and analysis
· Photomanipulation (optional): Compatible with the Photomanipulation (PM) module for FRAP, photoablation, optogenetics, and ROI-based stimulation

(IV) Producer: Bruker

(V) Aplications: Live-Cell Imaging, Developmental Biology, Cell Biology, Neuroscience, Organoid and 3D Cell Culture Research, Cancer Biology, Stem Cell Research, Calcium Imaging, Structured Illumination Microscopy (SIM), Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Protein Dynamics and Cell Signaling , Photomanipulation and Optogenetics, Long-Term Live Imaging.