Bruker Papilio 5D Light-Sheet Microscope

(I) Description:

The Bruker Papilio™ 5D is a next-generation light-sheet fluorescence microscope engineered for high-sensitivity, low-phototoxicity imaging of living biological specimens. Designed for applications ranging from organoids and 3D cell cultures to embryos and small model organisms, the system combines a horizontal optical geometry with dual-view detection and Gaussian or Bessel beam illumination to deliver deep, high-resolution imaging with exceptional flexibility. Integrated environmental control together with simultaneous multi-sample and multi-condition imaging enables long-term live-cell and in vivo studies while maintaining physiological conditions and sample integrity

(II) Features and benefits:

· Fast, gentle volumetric imaging with minimal photobleaching and phototoxicity
· Long-term live-cell imaging with high cell viability
· High-resolution 3D and time-lapse fluorescence imaging
· Simultaneous multi-color imaging of dynamic biological processes
· Integrated incubation for physiological imaging conditions
· Rapid acquisition of large volumetric datasets
· Flexible platform for cells, organoids, embryos, and small model organisms

(III) Technical Specifications:

· Imaging modality: Dual-view light-sheet fluorescence microscopy (LSFM)
· Illumination modes: Gaussian beam and Bessel beam
· Acquisition Modes: Area mode and confocal line mode
· Illumination objective: 20× / 0,6 NA
· Detection objectives: : Dual Nikon CFI APO LWD 25× / 1.10 NA or dual Nikon CFI APO LWD 16× / 0.80 NA
· Detection: Dual scientific CMOS cameras (Hamamatsu ORCA-Flash4.0 or ORCA-Fire)
· Magnification changer: Four discrete magnification steps
· Multi-sample imaging: Up to 6 independent experimental compartments
· Environmental control: Temperature (20–37 °C), CO₂, O₂, and humidity control for long-term live-cell imaging
· Data processing: Automated dual-view registration, fusion, destriping, and deconvolution in LuxBundle software
· Photomanipulation (optional): Compatible with CW and pulsed visible-to-NIR laser modules
· Software: Bruker LuxBundle for acquisition, visualization, and post-processing

(IV) Producer: Bruker

(V) Aplications: Live-Cell Imaging, In Vivo Imaging, Developmental Biology, Organoid and Spheroid Research, 3D Cell Culture Imaging, Stem Cell Research, Cancer Biology, Cell Biology, Neuroscience, Embryo Development, Small Model Organisms, Time-Lapse Imaging, Drug Discovery and Screening.