Bruker MuVi SPIM CS Light-Sheet Microscope

(I) Description:

Bruker MuVi SPIM CS is a high-performance multiview light-sheet fluorescence microscope designed for high-resolution imaging of large biological specimens. Its unique dual-sided illumination and detection architecture, combined with automated multiview image fusion, delivers near-isotropic resolution, excellent imaging depth, and outstanding image quality. The MuVi SPIM CS is ideally suited for developmental biology, cleared tissue imaging, neuroscience, and whole-organism studies.

(II) Features and benefits:

· Dual-sided illumination and dual-view detection for optimal image quality

· Automated multiview acquisition and image fusion for near-isotropic resolution

· Reduced shadowing and improved image uniformity throughout large specimens

· Fast volumetric imaging with minimal photobleaching and phototoxicity

· Deep imaging of cleared tissues, whole organs, embryos, and model organisms

· Large imaging volume with high spatial resolution

· Automated image reconstruction for accurate 3D visualization

· Flexible platform for developmental biology, neuroscience, tissue clearing, and spatial biology

(III) Technical Specifications:

• Imaging modality: Multiview Light-Sheet Fluorescence Microscopy (LSFM)
• Illumination geometry: Simultaneous dual-sided illumination
• Detection geometry: Dual-sided detection
• Image acquisition: Automated multiview imaging with image fusion
• Illumination mode: Digitally scanned Gaussian light sheet
• Detection objectives: Dual long-working-distance water immersion objectives
• Detection: Scientific CMOS (sCMOS) cameras
• Multi-view imaging: Four orthogonal views with automated sample rotation
• 3D reconstruction: Automated multiview registration and image fusion
• Software: Bruker LuxControl for acquisition and LuxBundle for image processing and visualization
• Sample compatibility: Cleared tissues, whole embryos, organoids, intact organs, and small model organisms

(IV) Producer: Bruker

(V) Aplications: Developmental biology, tissue clearing, neuroscience, whole-organ imaging, and spatial biology