Bruker Ultima Investigator Plus Multiphoton Microscope

(I) Description:

The Bruker Ultima Investigator Plus is a streamlined, modular multiphoton microscope optimized for live and intravital imaging with a large field of view and high-sensitivity deep-tissue detection. Its upgraded electronics architecture, enhanced collection path, and flexible add-on options support demanding applications ranging from functional neuroscience to immuno-oncology. The system can be expanded with three-photon imaging, high-speed scanning, multi-region acquisition, FLIM, optogenetics, and motorized positioning, allowing it to evolve with changing research requirements.

(II) Features & Benefits:

• Large field of view for imaging extended neural networks and tissue regions
• Wide-angle collection for improved signal-to-noise in deep, scattering samples
• High-sensitivity intravital imaging for functional and longitudinal studies
• Modular architecture with upgrade paths for three-photon imaging, FLIM, optogenetics, and high-speed acquisition
• Multi-region scanning for rapid acquisition from multiple targeted locations
• High-speed resonant scanning for dynamic functional imaging
• Flexible integration with behavioral paradigms and advanced neuroscience workflows
• Real-time access to imaging data for automated feedback and closed-loop experiments
• Upgradeable platform with a path toward Ultima 2Pplus capabilities

(III) Technical Specifications:

• Imaging modality: Multiphoton fluorescence microscopy optimized for live and intravital imaging
• Imaging configurations: Two-photon imaging with optional three-photon imaging capability
• Optical collection: Enhanced close-coupled, wide-angle detection path for efficient signal collection from scattering tissue
• Electronics: Bruker xCore electronics architecture for system-wide synchronization and modular expansion
• Field of view: Expanded large-field imaging; optional xView Module extends the imaging area by more than 2.5× compared with the standard field
• High-speed imaging: Optional resonant scanner providing approximately 30 fps at 512 × 512 pixels
• Region-of-interest imaging: More than 500 fps achievable with reduced ROI configurations
• Detection: Photomultiplier-based detection with optional high-sensitivity GaAsP detectors
• Z-positioning: Optional high-speed Z-piezo with 0.1 µm step size and 150 µm travel range
• Motorized XY stage: 6-inch X × 3-inch Y travel, with minimum 0.3 µm increments and approximately 1 µm positioning accuracy
• Multi-region scanning: Software-controlled acquisition from multiple defined regions
• FLIM (optional): TCSPC-based fluorescence lifetime imaging
• Photoactivation / photostimulation: Optional full-field or point/scanning photoactivation configurations
• Widefield imaging: Camera port for widefield epifluorescence detection
• Software: Bruker Prairie View for acquisition, Atlas Imaging, automation, external-device synchronization, and real-time experimental control
• Upgradeability: Field-upgradeable toward the Ultima 2Pplus platform and compatible with additional Bruker multiphoton modules

(IV) Manufacturer:

Bruker

(V) Applications:

In Vivo Brain Imaging, Intravital Microscopy, Functional Calcium Imaging, Neuroscience, Neural Circuit Imaging, Behavioral Neuroscience, Brain Mapping, Multi-Region Imaging, Deep-Tissue Imaging, Three-Photon Imaging, Immuno-Oncology, Tumor Imaging, Cancer Biology, Immune-Cell Imaging, Collagen Imaging, Longitudinal Imaging, FLIM, Optogenetics, Photoactivation, High-Speed Functional Imaging.