{"id":28479,"date":"2026-08-04T19:54:32","date_gmt":"2026-08-04T16:54:32","guid":{"rendered":"https:\/\/apellaser.ro\/?post_type=product&p=28479"},"modified":"2026-08-04T20:04:09","modified_gmt":"2026-08-04T17:04:09","slug":"platforma-de-multiplexare-plexflo","status":"publish","type":"product","link":"https:\/\/apellaser.ro\/en\/product\/platforma-de-multiplexare-plexflo\/","title":{"rendered":"Platforma de multiplexare PlexFlo"},"content":{"rendered":"

(I) Descriere<\/h2>\n

\u00cen domeniul microscopiei de localizare, tot mai multe aplica\u021bii necesit\u0103 imagistica simultan\u0103 a unui num\u0103r mare de \u021binte moleculare care dep\u0103\u0219e\u0219te capacitatea de separare spectral\u0103 a probelor\u00a0fluorescente conven\u021bionale. Marcarea secven\u021bial\u0103 cu ajutorul microfluidicii permite investigarea unui num\u0103r mult mai mare de \u021binte. Unit\u0103\u021bile microfluidice Bruker sunt concepute pentru a permite integrarea marc\u0103rii secven\u021biale \u00een protocoalele de microscopie de localizare utilizate cu microscopul Vutara VXL pentru localizarea moleculelor individuale.<\/p>\n

Bruker ofer\u0103 dou\u0103 unit\u0103\u021bi: PlexFlo<\/strong>, cu 15 rezervoare pentru reactivi, \u0219i PlexFlo96<\/strong>, cu 96 de rezervoare pentru reactivi \u0219i sistem de manipulare a reactivilor cu temperatur\u0103 controlat\u0103. Unit\u0103\u021bile sunt controlate prin software-ul SRX al sistemului Vutara \u0219i permit configurarea flexibil\u0103 a secven\u021belor microfluidice, care sunt executate \u00eempreun\u0103 cu ciclurile de imagistic\u0103. Astfel, utilizatorii pot marca un num\u0103r nelimitat de sonde \u0219i le pot imagina \u00een mod secven\u021bial.<\/p>\n

(II) Caracteristici \u0219i beneficii<\/h2>\n
    \n
  • Fluxuri de lucru automatizate de multiplexare pentru imagistic\u0103 de fluorescen\u021b\u0103 cu un grad ridicat de multiplexare.<\/li>\n
  • Marcarea secven\u021bial\u0103 a mai multor biomarkeri \u00een cadrul aceleia\u0219i probe.<\/li>\n
  • Sistem microfluidic programabil pentru administrarea automat\u0103 a reactivilor \u0219i efectuarea etapelor de sp\u0103lare.<\/li>\n
  • Integrare f\u0103r\u0103 \u00eentreruperi cu sistemul Bruker Vutara\u00ae VXL \u0219i software-ul SRX;<\/li>\n
  • Reducerea timpului de operare manual\u0103 prin fluxuri de lucru de imagistic\u0103 complet automatizate.<\/li>\n
  • Reproductibilitate ridicat\u0103 datorit\u0103 controlului precis al fluidelor prin presiune;<\/li>\n
  • Compatibilitate cu DNA-PAINT, Exchange-PAINT, OligoSTORM \u0219i alte tehnici de microscopie de localizare a moleculelor individuale \u2014 SMLM.<\/li>\n
  • Proiectarea flexibil\u0103 a protocoalelor pentru experimente personalizate de imagistic\u0103 multiplexat\u0103.<\/li>\n
  • Permite efectuarea analizelor avansate de biologie spa\u021bial\u0103 \u0219i de caracterizare a biomarkerilor.<\/li>\n<\/ul>\n

    (III) Specifica\u021bii tehnice<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Specifica\u021bie<\/th>\nPlexFlo<\/th>\nPlexFlo96<\/th>\n<\/tr>\n
    Probe\/rezervoare de volum mic<\/strong><\/td>\n15 flacoane \u00d7 2 ml, standard Eppendorf<\/td>\n96 de godeuri \u00d7 2 ml, \u00eentr-o microplac\u0103<\/td>\n<\/tr>\n
    Rezervoare de sp\u0103lare\/tampon, volum mare<\/strong><\/td>\n1 \u00d7 100 ml, 1 \u00d7 50 ml \u0219i 2 \u00d7 15 ml<\/td>\n8 recipiente GL 45 \u00d7 100 ml<\/td>\n<\/tr>\n
    Recipient pentru de\u0219euri<\/strong><\/td>\n1 recipient GL 45 \u00d7 100 ml<\/td>\n1 recipient GL 45 \u00d7 250 ml<\/td>\n<\/tr>\n
    Controlul temperaturii probei<\/strong><\/td>\nNu<\/td>\n39\u201399 \u00b0F \/ 4\u201337 \u00b0C, valoare de referin\u021b\u0103 pentru placa de baz\u0103 a micropl\u0103cii<\/td>\n<\/tr>\n
    \u00cenlocuirea probelor \u00een timpul experimentului<\/strong><\/td>\nDa<\/td>\nDa<\/td>\n<\/tr>\n
    Controlul debitului prin presiune<\/strong><\/td>\nDa<\/td>\nDa; include \u0219i vid pentru extragerea din placa cu 96 de godeuri<\/td>\n<\/tr>\n
    Interval de presiune<\/strong><\/td>\n0\u2013345 mbar; CV \u00b10,1% peste 10% din scala complet\u0103; rezolu\u021bie \u00b1105 \u03bcbar<\/td>\nDe la \u2212700 la 900 mbar<\/td>\n<\/tr>\n
    Senzor de debit<\/strong><\/td>\nSenzor termic, 1 \u03bcl\/min\u20131 ml\/min, CV \u00b11%<\/td>\nSenzor termic, 1 \u03bcl\/min\u20131 ml\/min; \u00b15% din valoarea m\u0103surat\u0103 peste 40 \u03bcl\/min \u0219i \u00b11,5 \u03bcl\/min sub 40 \u03bcl\/min<\/td>\n<\/tr>\n
    Integrare software<\/strong><\/td>\nDa<\/td>\nDa<\/td>\n<\/tr>\n
    Procedur\u0103 automat\u0103 de cur\u0103\u021bare<\/strong><\/td>\nDa<\/td>\nDa<\/td>\n<\/tr>\n
    Dimensiuni<\/strong><\/td>\n370 \u00d7 300 \u00d7 204 mm<\/td>\n520 \u00d7 430 \u00d7 420 mm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    (IV) Produc\u0103tor<\/h2>\n

    Bruker<\/strong><\/p>\n

    (V) Aplica\u021bii<\/h2>\n

    Biologie spa\u021bial\u0103 cu grad ridicat de multiplexare, Imagistic\u0103 DNA-PAINT, Cartografierea cromatinei, OligoSTORM, Imagistic\u0103 multiplexat\u0103 cu anticorpi, Hibridizare fluorescent\u0103 in situ la nivel de molecul\u0103 individual\u0103 \u2014 smFISH, Biologie celular\u0103, Biologia cancerului, Neuro\u0219tiin\u021be, Imunologie, Genomic\u0103,Proteomic\u0103 spa\u021bial\u0103.<\/p>\n","protected":false},"excerpt":{"rendered":"

    (I) Descriere \u00cen domeniul microscopiei de localizare, tot mai multe aplica\u021bii necesit\u0103 imagistica simultan\u0103 a unui num\u0103r mare de \u021binte<\/p>\n","protected":false},"featured_media":28480,"template":"","meta":[],"product_brand":[],"product_cat":[3573],"product_tag":[],"class_list":["post-28479","product","type-product","status-publish","has-post-thumbnail","product_cat-microscopie-de-super-rezolutie","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nPlatforma de multiplexare PlexFlo - APEL LASER<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/apellaser.ro\/en\/product\/platforma-de-multiplexare-plexflo\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Platforma de multiplexare PlexFlo - APEL LASER\" \/>\n<meta property=\"og:description\" content=\"(I) Descriere \u00cen domeniul microscopiei de localizare, tot mai multe aplica\u021bii necesit\u0103 imagistica simultan\u0103 a unui num\u0103r mare de \u021binte\" \/>\n<meta property=\"og:url\" content=\"https:\/\/apellaser.ro\/en\/product\/platforma-de-multiplexare-plexflo\/\" \/>\n<meta property=\"og:site_name\" content=\"APEL LASER\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/apellaser\" \/>\n<meta property=\"article:modified_time\" content=\"2026-08-04T17:04:09+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/apellaser.ro\/wp-content\/uploads\/2026\/08\/Picture2.png\" \/>\n\t<meta property=\"og:image:width\" content=\"624\" \/>\n\t<meta property=\"og:image:height\" content=\"360\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/apellaser.ro\\\/en\\\/product\\\/platforma-de-multiplexare-plexflo\\\/\",\"url\":\"https:\\\/\\\/apellaser.ro\\\/en\\\/product\\\/platforma-de-multiplexare-plexflo\\\/\",\"name\":\"Platforma de multiplexare PlexFlo - 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