Due to this versatility, CRONUS-2P can be applied for the simultaneous excitation of multiple fluorescent probes, calcium indicators, or opsins at their absorption maxima. Additionally, second- and third\u2011harmonic emission (SHG and THG) can be spectrally shifted for easier detection or resonant enhancement.<\/p>\n
The two tunable and simultaneous channels also enable advanced coherent anti-Stokes and stimulated Raman scattering (CARS and SRS) applications with dual-band imaging, a broader selection of vibrational resonance frequencies, constant-difference dual\u2011beam tuning, and resonant enhancement.<\/p>\n
CRONUS-2P is a three-channel tunable femtosecond laser providing watt-level, synchronized outputs with high repetition rates, short pulse durations, and GDD control, making it the ultimate source for nonlinear microscopy. In its standard configuration, the two independently tunable outputs, which can operate separately or simultaneously, cover 680\u00a0\u2013 960\u00a0nm and 940\u00a0\u2013 1300\u00a0nm, respectively. The third output, fixed at 1025\u00a0nm, is accessible in parallel.<\/p>\n